During the COVID-19 pandemic, teleworking affected the mental well-being of nearly all strata of the population. Teachers, who were employed to teach online courses during lockdown have been psychologically distressed. The primary aim of the current study was to estimate the prevalence and differences in the Burnout Syndrome (BOS) symptomatology in the light of gender, work position, teaching role, and subject taught. As a secondary aim, differences in perceived well being were estimated through the contribution of individual factors.